The order designates May 3 through May 4, 2025, as National Fallen Firefighters Memorial Weekend. It instructs that the flag of the United States be flown at half-staff at all Federal office buildings in honor of the National Fallen Firefighters Memorial Service on Sunday, May 4, 2025. The directive aims to honor volunteer and professional firefighters who have lost their lives in the line of duty over the past year.

The order directs federal agencies to establish the Religious Liberty Commission. This Commission, composed of up to 14 appointed members and ex officio members including the Attorney General, the Secretary of Housing and Urban Development, and the Assistant to the President for Domestic Policy, is tasked with producing a comprehensive report on the foundations of religious liberty in America, its impact on society, current threats to it, strategies to preserve and enhance protections for future generations, and programs to increase awareness of and celebrate America’s peaceful religious pluralism. The Commission will also examine specific areas such as First Amendment rights, attacks on houses of worship, and parents’ authority over their children's education.

The proclamation designates April 29, 2025, as a day to commemorate the 418th anniversary of the first landing and the raising of the Cape Henry Cross by the Jamestown settlers in Virginia. It encourages the nation to remember and honor the historical event and the settlers' dedication to their faith and their new homeland.

The proclamation recognizes April 26, 2025 as World Intellectual Property Day. It emphasizes the importance of safeguarding American intellectual property and innovation, and encourages citizens to celebrate the achievements of creators and inventors. The proclamation also mentions past actions to protect intellectual property, including the establishment of the Council of Advisors on Science and Technology, the use of tariffs to secure stronger intellectual property protections in trade deals, and an executive order aimed at removing barriers to American leadership in artificial intelligence.

The proclamation calls for the observance of the Days of Remembrance of Victims of the Holocaust from April 20 through April 27, 2025. It encourages the public to commemorate the victims of the Holocaust and Nazi persecution through study, prayers, and remembering the lessons of this atrocity to prevent its repetition. It also instructs the Federal Government to use all available and appropriate legal tools to combat anti-Semitic harassment in schools and on college campuses.

The proclamation designates April 20 through April 26, 2025, as National Volunteer Week. It encourages all Americans to participate in volunteer service projects across the country and to make service a regular part of their lives.

The order directs Federal agencies to recognize April 19, 2025, as a day of commemoration for the 250th anniversary of the Battles of Lexington and Concord, marking the beginning of the American Revolutionary War. This commemoration is intended to honor the sacrifices made by those who fought in these battles, and to renew commitment to the principles of independence and self-government for which they stood. The directive does not specify actions to be taken by individual agencies, but implies a general expectation of acknowledgement and commemoration.

The order directs Federal agencies to recognize April 2025 as National Donate Life Month. It encourages all eligible individuals, regardless of age or medical history, to consider becoming organ donors and share this decision with family and loved ones. The order also mentions the option to register through state registries, the Department of Motor Vehicles, or www.organdonor.gov.

The order directs Federal agencies to recognize April 2025 as National Sexual Assault Awareness and Prevention Month. It urges all Americans, families, law enforcement personnel, healthcare providers, and community and faith-based organizations to support survivors of sexual assault and work together to prevent such crimes. The order also highlights previous actions taken, such as the declaration of a national emergency at the southern border, designation of cartels as terrorist organizations, and the signing into law of the Laken Riley Act requiring detention of illegal aliens convicted of certain crimes.