The order directs Federal agencies and agency subdivisions, including the Departments of State, Defense, Treasury, Veterans Affairs, Justice, Health and Human Services, Homeland Security, the Interior, Energy, Agriculture, Commerce, the Environmental Protection Agency, the United States Agency for International Development, the Nuclear Regulatory Commission, the National Science Foundation, the United States International Trade Commission, the Federal Communications Commission, the General Services Administration, and others, to exclude themselves from Federal Labor-Management Relations Programs. This exclusion is based on the determination that these agencies and subdivisions primarily function in intelligence, counterintelligence, investigative, or national security work, and that the application of certain sections of the United States Code to these entities is inconsistent with national security requirements and considerations. Specific exclusions are

The order directs Federal agencies to receive nominations for various positions. The Department of the Army is to consider Marc Andersen, William Gillis, and Jules Hurst III for the role of Assistant Secretary. The National Aeronautics and Space Administration and the Department of Labor are to consider Gregory Autry and David Brian Castillo respectively for the position of Chief Financial Officer. Donald Bergin III is nominated for Assistant Secretary of Veterans Affairs (Congressional and Legislative Affairs), Marc Berkowitz for Assistant Secretary of Defense, and Jonathan Berry for Solicitor for the Department of Labor. The Department of Labor is also to consider Anthony D’Esposito for Inspector General and Andrew Rogers for Administrator of the Wage and Hour Division. The Navy is to consider Benjamin Kohl

The order directs federal agencies to prepare Americans for high-paying skilled trade jobs of the future. The Secretaries of Labor, Commerce, and Education are instructed to review all Federal workforce development programs and submit a strategy to optimize these programs, with a focus on in-demand skills of emerging industries. Additionally, these agencies are tasked with expanding Registered Apprenticeships and improving transparency on the performance outcomes of workforce development programs. They are given 90 days to report on strategies to help American workers and 120 days to plan for expanding apprenticeships.

The order directs federal agencies to enforce English proficiency requirements for commercial vehicle operators. The Secretary of Transportation, through the Federal Motor Carrier Safety Administration (FMCSA), is instructed to rescind a 2016 guidance document and issue new guidance to ensure compliance with English language proficiency requirements. The FMCSA is also directed to review non-domiciled commercial driver’s licenses for irregularities and improve verification of driving credentials. Additionally, the Secretary of Transportation is tasked with identifying actions to improve the working conditions of truck drivers.

The order directs federal agencies to exclude additional units from the Federal Labor-Management Relations Program, citing national security reasons. Agencies and subdivisions including units in the Bureau of Reclamation with primary responsibility for hydropower facilities, the International Trade Administration, the Office of the Commissioner for Patents and subordinate units in the Patent and Trademark Office, certain subdivisions of the National Oceanic and Atmospheric Administration, the National Aeronautics and Space Administration, and the United States Agency for Global Media are affected. The order also extends the deadline for any order published by the Secretaries of Defense and Veterans Affairs related to exclusions from Federal Labor-Management Relations Programs.

The proclamation designates September 1, 2025, as Labor Day, calling for all public officials and people of the United States to honor the contributions and resilience of working Americans with appropriate programs, ceremonies, and activities. It also highlights the administration's efforts to restore the dignity of labor, protect American jobs, and ensure fair trade deals. The proclamation mentions the amassing of hundreds of billions of dollars in tariff revenue and the creation of new jobs at a record pace.

The order directs federal agencies to establish an emergency board to investigate disputes between the Long Island Rail Road Company and its employees represented by various labor organizations, including the Transportation Communications Union, the Brotherhood of Locomotive Engineers and Trainmen, the Brotherhood of Railroad Signalmen, the International Association of Machinists and Aerospace Workers, and the International Brotherhood of Electrical Workers. The board, composed of a chair and two other members, is to be appointed to investigate and report on these disputes. During the board's 120-day operation, no changes to the conditions causing the disputes can be made unless agreed upon by the parties involved.

The order directs federal agencies to address the misuse of the H-1B non-immigrant visa program, which has been exploited to replace American workers with lower-paid, lower-skilled labor, particularly in IT and STEM fields. It highlights the significant increase in foreign STEM workers from 1.2 million in 2000 to nearly 2.5 million in 2019, largely facilitated by the abuse of the H-1B visa. The order also raises concerns about the impact on American IT workers, with unemployment rates increasing from 1.98% in 2019 to 3.02% in 2025, and reports of American tech companies laying off local workers while hiring thousands of H-1B workers.

The order directs the establishment of a second emergency board to investigate unresolved disputes between the Long Island Rail Road Company and several of its employee labor organizations. The labor organizations involved are the Transportation Communications Union, the Brotherhood of Locomotive Engineers and Trainmen, the Brotherhood of Railroad Signalmen, the International Association of Machinists and Aerospace Workers, and the International Brotherhood of Electrical Workers. The second emergency board, comprised of a chair and two other members, will be appointed to investigate and report on these disputes.